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Assessment of Kidney Function in Mouse Models of Glomerular Disease
Published on: June 30, 2018
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Detailed process analysis for glomerular capillary formation by immunofluorescence on ultra-thick sections
Ting Yu1, Fang Zhang2, Yan Wu2
1Department of Nephrology, Institute of Nephrology of Chongqing and Kidney Center of PLA, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China; Department of Respiratory Medicine, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China.
Gene Expression Patterns : GEP
|February 7, 2020
Summary
This study reveals how glomerular capillaries form in mouse kidneys, detailing their expansion via proliferation, sprouting, and intussusceptive angiogenesis. Key genes like VEGFA and CXCR4 are crucial for this complex developmental process.
Area of Science:
- Renal developmental biology
- Vascular biology
- Cell biology
Background:
- Glomerular capillary formation is a poorly understood process in kidney development.
- Existing knowledge lacks detailed clarification on the mechanisms involved.
Purpose of the Study:
- To elucidate the detailed process of glomerular capillary formation in embryonic and postnatal mouse kidneys.
- To identify key molecular players involved in this developmental pathway.
Main Methods:
- Antibody staining on ultra-thick sections of embryonic and postnatal mouse kidneys.
- Analysis of gene expression patterns.
- Immunofluorescence microscopy.
Main Results:
- Kidney blood vessels expand through proliferation and sprouting.
- Capillaries bud and migrate into the glomerular cleft during comma and S-shaped stages.
- Intussusceptive angiogenesis drives capillary bed expansion, followed by arteriole pruning.
- VEGFA distribution in nephron epithelial cells and CXCR4 signaling are critical for capillary development.
Conclusions:
- The study clarifies the multi-step process of glomerular capillary formation, involving sprouting, intussusceptive angiogenesis, and pruning.
- VEGFA and CXCR4 are identified as key regulators of glomerular capillary development.
- Ultra-thick section immunofluorescence is an effective method for studying complex tissue development.
