Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A DNA-modification methylase from Bacillus stearothermophilus V.

R Barra1, M Chiong, E González

  • 1Laboratorio de Biologia Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago.

The Biochemical Journal
|October 15, 1988
PubMed
Summary

Researchers purified a type II modification methylase (M BstVI) from Bacillus stearothermophilus V. This enzyme transfers methyl groups to DNA, specifically methylating adenine in the 5'-C-T-C-G-A-G-3' sequence, protecting it from restriction enzymes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Neuroanatomical mapping of inhibitory attention and working memory with functional magnetic resonance imaging in healthy children].

Revista de neurologia·2024
Same author

Anaerobic RSH-dependent tellurite reduction contributes to Escherichia coli tolerance against tellurite.

Biological research·2022
Same author

Understanding gold toxicity in aerobically-grown Escherichia coli.

Biological research·2020
Same author

The effect of aromatherapy with lavender (Lavandula angustifolia) on serum melatonin levels.

Complementary therapies in medicine·2019
Same author

An approach to utilize the artificial high power LED UV-A radiation in photoreactors for the degradation of methylene blue.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2016
Same author

Effect of gamma irradiation on phenol content, antioxidant activity and biological activity of black maca and red maca extracts (Lepidium meyenii walp).

Toxicology mechanisms and methods·2015

Area of Science:

  • Molecular Biology
  • Enzymology
  • Thermophilic Bacteria

Background:

  • Type II modification methylases play crucial roles in DNA metabolism and protection.
  • Bacillus stearothermophilus V is a thermophilic bacterium with unique enzymatic properties.

Purpose of the Study:

  • To partially purify and characterize a type II modification methylase (M BstVI) from Bacillus stearothermophilus V.
  • To identify the substrate and methylation site of the M BstVI methylase.

Main Methods:

  • Partial purification of M BstVI from Bacillus stearothermophilus V.
  • Enzymatic assays measuring methyl group transfer from S-adenosyl-L-methionine to DNA.
  • Paper chromatography to identify the methylation product.
  • Analysis of DNA protection against restriction endonucleases (BstVI and XhoI).

Related Experiment Videos

Main Results:

  • M BstVI was partially purified from the thermophilic bacterium Bacillus stearothermophilus V.
  • The methylase catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to double-stranded DNA.
  • The product of methylation was identified as N6-methyladenine.
  • M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases.
  • The methylation occurs at the adenine residue within the 5 omino-C-T-C-G-A-G-3 omino sequence.

Conclusions:

  • M BstVI is a type II modification methylase from Bacillus stearothermophilus V.
  • The enzyme specifically methylates adenine in the 5 omino-C-T-C-G-A-G-3 omino recognition site.
  • This methylation confers resistance to BstVI and XhoI restriction endonucleases.