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Isolation and primary structure of human peptide YY
1Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, California.
Biochemical and Biophysical Research Communications
|December 15, 1988
Summary
Researchers isolated and synthesized human peptide YY (PYY), a 36-amino acid peptide. They determined its primary structure and confirmed synthetic PYY matches the natural form, aiding further research.
Area of Science:
- Biochemistry
- Endocrinology
- Peptide Chemistry
Background:
- Peptide YY (PYY) is a gastrointestinal hormone involved in regulating appetite and gut function.
- Understanding the precise structure of human PYY is crucial for studying its biological activity and developing therapeutic applications.
Purpose of the Study:
- To isolate and determine the primary structure of human peptide YY (PYY).
- To chemically synthesize human PYY and verify its structural identity with the natural peptide.
Main Methods:
- Purification of human PYY from colonic extracts using chemical methods.
- Determination of the complete 36-amino acid sequence of human PYY.
- Solid-phase synthetic technique for chemical synthesis of human PYY.
Main Results:
- Human PYY was isolated and its complete amino acid sequence determined.
- Structural differences were identified between human and porcine PYY at positions 3 and 18.
- Synthetic human PYY was found to be structurally identical to the naturally isolated peptide.
Conclusions:
- The primary structure of human PYY has been elucidated.
- Chemical synthesis provides a reliable method for obtaining structurally identical human PYY.
- This structural information facilitates further investigation into PYY's physiological roles.