Un-steady state modeling for free cyanide removal and biofilm growth in a RBC batch process

Biofilm growth and free cyanide biological removal from gold mine wastewater were modeled and simulated using a bench-scale rotating biological contactor (RBC). Eight batch cultures were run in three independent compartments (1.7 L, each) of the RBC. The system worked under the following conditions: [CN_i^-] = 0.3 g/L, pH = 10.5 ± 0.5, T = 20 ± 5 °C, ω =5 rpm, and 40.5 % of disc submersion. During each culture, biofilm thickness, biomass, and free cyanide concentration in the liquid were quantified. Subsequently, μ_max, [Formula: see text] , [Formula: see text] were determined using experimental data to later model and simulate the biofilm thickness and free cyanide biological removal with Wolfram Mathematica software. After the experiments, free cyanide biological removal was 96.33 % after three days, and maximum biofilm thickness was 0.0292 cm in the 16th day. Moreover, biofilm growth and free cyanide consumption models were adjusted to the experimental data with r² = 0.90 and r² = 0.99. Also, there was an equivalent error of 7.89 and 7.38 and a standard deviation of 10.89 % and 10.17 %, between the models and their experimental data, respectively. Finally, the proposed models will allow improvement of reactor operation and its design.