Assessing intracellular pH regulation in H⁺-ATPase-rich ionocytes in zebrafish larvae using in vivo ratiometric...

The H⁺-ATPase-rich (HR) cells of zebrafish larvae are a sub-type of ion-transporting cell located on the yolk sac epithelium that are responsible for Na⁺ uptake and H⁺ extrusion. Current models of HR cell ion transport mechanisms in zebrafish larvae are well established, but little is known about the involvement of the various ion transport pathways in regulating intracellular acid-base status. Here, a ratiometric imaging technique was developed and validated to monitor intracellular pH (pHi) continuously in larval zebrafish HR cells in vivo Gene knockdown or CRISPR/Cas9 knockout approaches were used to evaluate the roles of the two principal apical membrane acid excretory pathways, the Na⁺/H⁺ exchanger (NHE3b; slc9a3.2) and the H⁺-ATPase (atpv1aa). Additionally, the role of HR cell cytosolic carbonic anhydrase (CAc) was investigated because of its presumed role in providing H⁺ for Na⁺/H⁺ exchange and H⁺-ATPase. The temporal pattern and extent of intracellular acidification during exposure of fish to 1% CO₂ and the extent of post-CO₂ alkalisation were altered markedly in fish experiencing knockdown/knockout of CAc, NHE3b or H⁺-ATPase. Although there were slight differences among the three knockdown/knockout experiments, the typical response was a greater degree of intracellular acidification during CO₂ exposure and a reduced capacity to restore pHi to baseline levels post-hypercapnia. The metabolic alkalosis and subsequent acidification associated with 20 mmol l^-1 NH₄Cl exposure and its washout were largely unaffected by gene knockdown. Overall, the results suggest markedly different mechanisms of intracellular acid-base regulation in zebrafish HR cells depending on the nature of the acid-base disturbance.