Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

97.4K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.4K
DNA Microarrays02:34

DNA Microarrays

20.4K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improved understanding of sequence polymorphisms at 42 Y chromosome short tandem repeats for the Chinese Han population.

Forensic science international. Genetics·2024
Same author

Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs.

International journal of legal medicine·2024
Same author

Developmental validation of a high-resolution panel genotyping 639 Y-chromosome SNP and InDel markers and its evolutionary features in Chinese populations.

BMC genomics·2023
Same author

Application of Duplex Droplet Digital PCR Detection of miR-888 and miR-891a in Semen Identification.

Fa yi xue za zhi·2023
Same author

Evaluation of the MHSeqTyper47 kit for forensically challenging DNA samples.

Forensic science international. Genetics·2022
Same author

Sequence polymorphisms of forensic Y-STRs revealed by a 68-plex in-house massively parallel sequencing panel.

Forensic science international. Genetics·2022

Related Experiment Video

Updated: Dec 29, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.5K

A 124-plex Microhaplotype Panel Based on Next-generation Sequencing Developed for Forensic Applications.

Jing-Bo Pang1,2,3, Min Rao1,2,3, Qing-Feng Chen1,2

  • 1National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, PR China.

Scientific Reports
|February 8, 2020
PubMed
Summary

This study introduces a 124-plex microhaplotype panel for advanced forensic genetics. Microhaplotypes offer superior mixture deconvolution compared to short tandem repeats (STRs), enhancing individual identification in forensic investigations.

More Related Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.5K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

22.8K

Related Experiment Videos

Last Updated: Dec 29, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.5K
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.5K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

22.8K

Area of Science:

  • Forensic Genetics
  • Next-Generation Sequencing (NGS)

Background:

  • Microhaplotypes are emerging forensic genetic markers with significant potential.
  • Current methods like short tandem repeats (STRs) have limitations in complex forensic scenarios.

Purpose of the Study:

  • To develop and validate a 124-plex microhaplotype genotyping panel using NGS.
  • To evaluate the performance of microhaplotypes against STRs for forensic applications, particularly mixture deconvolution.

Main Methods:

  • Development of a 124-plex microhaplotype panel for NGS.
  • Genotyping of DNA from Han Chinese individuals (n=256).
  • Comparative analysis with capillary electrophoresis-based STRs and NGS-based microhaplotype genotyping.

Main Results:

  • The 124-plex panel provided balanced sequencing data and accurate genotypes from low DNA input (0.1 ng).
  • Microhaplotypes demonstrated superior performance in mixture deconvolution compared to STRs.
  • Forensic parameters indicated microhaplotypes are as effective as STRs for individual identification, with a combined match probability of 5.23 × 10-66 for 106 independently hereditary markers.

Conclusions:

  • The developed 124-plex microhaplotype panel is a powerful tool for forensic genetic analysis.
  • Microhaplotypes offer significant advantages over STRs, especially for complex mixture samples.
  • This panel enhances the capabilities of forensic investigations through improved individual identification and mixture analysis.