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Selective autophagy degrades nuclear pore complexes
Chia-Wei Lee1, Florian Wilfling1, Paolo Ronchi2
1Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
Nature Cell Biology
|February 8, 2020
Summary
Nuclear pore complexes (NPCs) undergo degradation via autophagy, a cellular process. This mechanism, involving specific proteins like Nup159, allows for the removal of individual NPCs from the nuclear envelope.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Nuclear pore complexes (NPCs) are large protein assemblies regulating transport between the nucleus and cytoplasm.
- Disruption of NPC integrity is implicated in aging, cancer, and neurodegenerative diseases.
- The mechanism for NPC turnover remains largely unknown.
Purpose of the Study:
- To investigate the mechanism of nuclear pore complex (NPC) turnover.
- To identify the cellular machinery involved in NPC degradation.
Main Methods:
- Utilized nitrogen starvation and genetic manipulation in Saccharomyces cerevisiae.
- Investigated the role of vacuolar proteases and autophagy.
- Analyzed the interaction between nucleoporins and autophagy components.
Main Results:
- NPCs are rapidly degraded following nitrogen starvation or architectural disruption.
- NPC turnover involves vacuolar proteases and the core autophagy machinery.
- Nup159 acts as an intrinsic cargo receptor, directly binding Atg8 for autophagic degradation.
Conclusions:
- Autophagic degradation is an inducible mechanism for NPC removal.
- Nup159 mediates the autophagic turnover of NPCs.
- This study elucidates a novel pathway for NPC quality control and removal.
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