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Related Concept Videos

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Regulation of Nuclear Protein Sorting01:45

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Autophagy01:27

Autophagy

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
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Nuclear Export01:42

Nuclear Export

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The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
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Autophagic Cell Death01:18

Autophagic Cell Death

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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
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Related Experiment Video

Updated: Dec 29, 2025

Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy
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Selective autophagy degrades nuclear pore complexes.

Chia-Wei Lee1, Florian Wilfling1, Paolo Ronchi2

  • 1Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Nature Cell Biology
|February 8, 2020
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Summary
This summary is machine-generated.

Nuclear pore complexes (NPCs) undergo degradation via autophagy, a cellular process. This mechanism, involving specific proteins like Nup159, allows for the removal of individual NPCs from the nuclear envelope.

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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclear pore complexes (NPCs) are large protein assemblies regulating transport between the nucleus and cytoplasm.
  • Disruption of NPC integrity is implicated in aging, cancer, and neurodegenerative diseases.
  • The mechanism for NPC turnover remains largely unknown.

Purpose of the Study:

  • To investigate the mechanism of nuclear pore complex (NPC) turnover.
  • To identify the cellular machinery involved in NPC degradation.

Main Methods:

  • Utilized nitrogen starvation and genetic manipulation in Saccharomyces cerevisiae.
  • Investigated the role of vacuolar proteases and autophagy.
  • Analyzed the interaction between nucleoporins and autophagy components.

Main Results:

  • NPCs are rapidly degraded following nitrogen starvation or architectural disruption.
  • NPC turnover involves vacuolar proteases and the core autophagy machinery.
  • Nup159 acts as an intrinsic cargo receptor, directly binding Atg8 for autophagic degradation.

Conclusions:

  • Autophagic degradation is an inducible mechanism for NPC removal.
  • Nup159 mediates the autophagic turnover of NPCs.
  • This study elucidates a novel pathway for NPC quality control and removal.