Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

703
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
703
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PD-1 Imposes Qualitative Control of Cellular Transcriptomes in Response to T Cell Activation.

Molecular cell·2020
Same author

MicroRNA-140 mediates RB tumor suppressor function to control stem cell-like activity through interleukin-6.

Oncotarget·2017
Same author

Loss of function mutations in the rice chromomethylase OsCMT3a cause a burst of transposition.

The Plant journal : for cell and molecular biology·2015
Same author

Temperature insensitive single-mode-multimode-single-mode fiber optic structures with two multimode fibers in series.

Optics letters·2014
Same author

Phosphorylation of multifunctional nucleolar protein nucleophosmin (NPM1) by aurora kinase B is critical for mitotic progression.

FEBS letters·2014
Same author

Odontogenic myxoma in a 52-year-old woman.

BMJ case reports·2014
Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: Dec 29, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K

Common-path multimodal three-dimensional fluorescence and phase imaging system.

Manoj Kumar1, Xiangyu Quan1, Yasuhiro Awatsuji2

  • 1Kobe Univ., Japan.

Journal of Biomedical Optics
|February 8, 2020
PubMed
Summary
This summary is machine-generated.

A new multimodal system combines coherent and incoherent digital holographic microscopes (DHMs) for simultaneous 3-D phase and fluorescence imaging of biological specimens. This stable, compact system offers enhanced understanding of biological processes and diseases.

Keywords:
common-path configurationdigital holographymultimodalthree-dimensional fluorescence imagingthree-dimensional phase imaging

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.2K
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

948

Related Experiment Videos

Last Updated: Dec 29, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.2K
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

948

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Biophysics

Background:

  • Digital holographic microscopy (DHM) offers 3-D imaging capabilities.
  • Simultaneous phase and fluorescence imaging is crucial for understanding biological dynamics.
  • Existing multimodal systems can be complex and sensitive to vibrations.

Purpose of the Study:

  • To develop a stable multimodal system for simultaneous 3-D phase and fluorescence imaging.
  • To combine coherent and incoherent DHMs in a common-path configuration.
  • To analyze biological specimens with high spatial and temporal resolution.

Main Methods:

  • A single-shot common-path off-axis fluorescent DHM was used for 3-D fluorescence imaging.
  • A stable common-path off-axis DHM utilizing a beam splitter was employed for phase imaging.
  • The system was tested on fluorescent microspheres and living moss cells.

Main Results:

  • The multimodal system successfully achieved simultaneous 3-D phase and fluorescence imaging.
  • The common-path geometry ensured high temporal phase stability and reduced vibration sensitivity.
  • Experiments demonstrated the system's capability for live imaging of fluorescent beads.

Conclusions:

  • The developed multimodal DHM system is feasible and stable.
  • It enables simultaneous 3-D phase and fluorescence imaging of biological specimens.
  • The system has potential applications in analyzing biological behaviors and understanding diseases.