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Updated: Dec 29, 2025

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The role of nucleolar spindle-associated protein 1 in human ovarian cancer

Yuyang Zhang1, Kate Huang2, Huihua Cai3

  • 1Department of Gynecology, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Journal of Cellular Biochemistry
|February 8, 2020
PubMed

Insights

Nucleolar spindle-associated protein 1 (NuSAP1) is highly expressed in ovarian cancer (OC). Inhibiting NuSAP1 in OC cells suppresses proliferation and metastasis, suggesting NuSAP1 as a potential therapeutic target for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Ovarian cancer (OC) is a leading cause of cancer-related deaths in women.
  • Understanding the molecular mechanisms driving OC progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of Nucleolar spindle-associated protein 1 (NuSAP1) in the development and progression of ovarian cancer.
  • To evaluate NuSAP1 as a potential prognostic marker and therapeutic target for OC.

Main Methods:

  • NuSAP1 expression was analyzed in OC tissues and cell lines.
  • NuSAP1 was knocked down using lentivirus-mediated shRNA.
  • Cell proliferation, apoptosis, cell cycle, migration, and invasion were assessed using MTT, colony formation, flow cytometry, and Transwell assays.

Main Results:

  • NuSAP1 was found to be highly expressed in OC tissues and cell lines.
  • NuSAP1 knockdown significantly inhibited OC cell proliferation and colony formation.
  • NuSAP1 knockdown promoted apoptosis, altered cell cycle distribution, and suppressed migration and invasion in OC cells.

Conclusions:

  • NuSAP1 functions as an oncogenic factor in ovarian cancer.
  • NuSAP1 may serve as a valuable prognostic indicator and a potential therapeutic target for OC treatment.
Keywords:
NuSAP1apoptosismetastasisovarian cancerproliferation

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