Evaluation of DNA damage and toxicological methodology development: A bibliometric study
F A Nascimento1, D de Melo E Silva1, H F Nunes1
1Laboratório de Mutagênese (LabMut), Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Brazil.
Human & Experimental Toxicology
|February 8, 2020
Summary
The comet assay (CA) is a sensitive tool for detecting DNA damage and genomic instability. Research shows its versatility, with China leading recent advancements, particularly in oxidative damage studies.
Area of Science:
- Biotechnology
- Genetics
- Toxicology
Background:
- Genomic instability poses risks to organism health.
- The comet assay (CA) is a highly sensitive and versatile method for detecting DNA damage.
- CA is applicable to genotoxicity analysis of drugs and carcinogens.
Purpose of the Study:
- To assess the scientific importance, employability, and trends of the comet assay (CA).
- Analysis of papers in the Scopus database from 1990 to 2018.
Main Methods:
- Bibliometric analysis of 13,828 articles.
- Evaluation of publication trends, citations, author clusters, countries, journals, and impact factors (IF) CiteScore.
- Identification of thematic clusters and correlation analysis.
Main Results:
- A peak in CA publications occurred in 2014, followed by a decline.
- Four major author clusters were identified: China, United States, India, and Brazil.
- China shows the most recent scientific advances, with increased use in oxidative damage research.
- Positive correlations were found between publications, citations, and IF.
Conclusions:
- The comet assay (CA) demonstrates full employability and versatility in DNA damage analysis.
- Chinese scientific production in this field is advancing rapidly.
- The method is increasingly utilized in oxidative damage research.
Related Concept Videos
01:25Mutagenicity and Carcinogenicity
1.8K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.8K
02:36DNA Damage can Stall the Cell Cycle
9.9K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K
02:25Overview of DNA Repair
33.2K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Chemically...
33.2K