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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

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Published on: September 25, 2018

Multiplex PCR implementation as point-of-care testing in a French emergency department

D Bouzid¹, J-C Lucet², X Duval³

¹AP-HP, Bichat Claude-Bernard Hospital, Emergency Department, Paris, France; Université de Paris, IAME, INSERM, Paris, France.

The Journal of Hospital Infection

|February 8, 2020

Summary

No abstract available in PubMed .

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