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Tailoring cellular metabolism in lactic acid bacteria through metabolic engineering
Anshula Sharma1, Gaganjot Gupta1, Tawseef Ahmad1
1Department of Biotechnology, Punjabi University, Patiala 147002, India.
Journal of Microbiological Methods
|February 8, 2020
Summary
Metabolic engineering enhances lactic acid bacteria (LAB) for industrial metabolite production. This review overviews strategies for tailoring LAB strains, leveraging their GRAS status and well-understood genetics for efficient biomanufacturing.
Area of Science:
- Biotechnology and Synthetic Biology
- Microbial Engineering
- Industrial Microbiology
Background:
- Lactic acid bacteria (LAB) are widely used in food, fermentation, and pharmaceutical industries due to their Generally Recognized As Safe (GRAS) status.
- Their well-understood genetics and metabolic regulation make them ideal hosts for producing valuable industrial compounds.
- Metabolic engineering, integrating synthetic and systems biology, offers powerful tools for pathway optimization.
Purpose of the Study:
- To provide a concise overview of metabolic engineering strategies applicable to lactic acid bacteria (LAB).
- To highlight the potential of LAB as robust expression systems for industrial metabolite production.
- To guide the comprehensive tailoring of LAB strains for large-scale biomanufacturing.
Main Methods:
- Review of current metabolic engineering techniques, including genetic manipulation and pathway reconstruction.
- Analysis of advancements in synthetic and systems biology approaches for microbial strain improvement.
- Examination of strategies for enhancing the efficiency and competitiveness of LAB-based production systems.
Main Results:
- Successful implementation of metabolic engineering strategies in various LAB strains has been demonstrated.
- Tailoring of cellular metabolism in LAB leads to highly efficient and industrially viable expression systems.
- LAB strains can be effectively engineered for the production of diverse industrially important metabolites.
Conclusions:
- Metabolic engineering provides a robust framework for optimizing LAB for industrial applications.
- Advancements in genetic tools and biological understanding enable precise control over LAB metabolism.
- Engineered LAB strains represent a promising platform for sustainable and cost-effective bioproduction of valuable compounds.
Keywords:
Biosynthetic pathwaysCentral carbon metabolismHeterologous gene expressionMetabolite productionSynthetic metabolic engineeringSystems biologyMore Related Videos
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