Related Experiment Video

Updated: Dec 29, 2025

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

10.9K

RNA-seq based transcriptome analysis of EHMT2 functions in breast cancer

Kwangho Kim1, Tae Young Ryu1, Jea-Woon Ryu1

  • 1Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.

Biochemical and Biophysical Research Communications
|February 9, 2020
PubMed

Insights

EHMT2 shows potential as a therapeutic target for all breast cancer subtypes, including triple-negative breast cancer (TNBC). This study identified EHMT2

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Hormone therapy is limited to hormone receptor-positive breast cancer.
  • Triple-negative breast cancer (TNBC) lacks targeted therapies.
  • Identifying novel therapeutic targets for all breast cancer subtypes is crucial.

Purpose of the Study:

  • To investigate the subtype-specific functions of EHMT2 in breast cancer.
  • To evaluate EHMT2 as a potential therapeutic target for all breast cancer subtypes.

Main Methods:

  • Established an RNA-sequencing (RNA-seq) analytical pipeline.
  • Performed EHMT2 knockdown in MB231 (TNBC) and MCF7 (hormone receptor-positive) cell lines.
  • Analyzed differentially expressed genes (DEGs) using Gene Ontology (GO), Gene Set Enrichment Analysis (GSEA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Main Results:

  • Identified subtype-specific and overlapping DEGs after EHMT2 knockdown.
  • Uncovered distinct functional roles of EHMT2 in MB231 and MCF7 cells.
  • EHMT2 influences key cellular pathways relevant to breast cancer progression.

Conclusions:

  • EHMT2 exhibits subtype-specific functions in breast cancer.
  • EHMT2 represents a promising therapeutic target for both hormone receptor-positive and triple-negative breast cancer.
  • Targeting EHMT2 could offer a treatment strategy for all breast cancer subtypes.
Keywords:
Breast cancerEHMT2RNA-seq

More Related Videos

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.6K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.5K

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K