Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nephrotic Syndrome I : Introduction01:24

Nephrotic Syndrome I : Introduction

476
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
476
Glomerular Filtration01:15

Glomerular Filtration

4.7K
The filtration membrane in the renal system is a highly specialized structure essential for filtering blood. It consists of glomerular capillaries and podocytes, forming a selective barrier that permits the passage of water and small solutes while restricting most plasma proteins and blood cells.
Components of the Filtration Membrane
The filtration process involves three key layers: the glomerular endothelial cells, the basement membrane, and the podocyte-formed filtration slits.
4.7K
Renal Corpuscle01:20

Renal Corpuscle

6.9K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
6.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Nephrons01:10

Nephrons

6.3K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
6.3K
Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

5.0K
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medical students' perception of what embodies an effective surgeon educator.

American journal of surgery·2021
Same author

Population-based comparison of prognostic factors in invasive micropapillary and invasive ductal carcinoma of the breast.

British journal of cancer·2014
Same author

A current approach to statin intolerance.

Clinical pharmacology and therapeutics·2014
Same author

Deriving indicators for breast conserving surgery using finite element analysis.

Computer methods in biomechanics and biomedical engineering·2013
Same author

Video. Chopstick surgery: a novel technique enables use of the Da Vinci Robot to perform single-incision laparoscopic surgery.

Surgical endoscopy·2010
Same author

How does RNA editing affect dsRNA-mediated gene silencing?

Cold Spring Harbor symposia on quantitative biology·2007

Related Experiment Video

Updated: Jan 11, 2026

Nephrotic Syndrome I : Introduction
01:24

Nephrotic Syndrome I : Introduction

Published on: June 19, 2025

476

An unwinding activity that covalently modifies its double-stranded RNA substrate.

B L Bass1, H Weintraub

  • 1Hutchinson Cancer Research Center, Seattle, Washington 98104.

Cell
|December 23, 1988
PubMed
Summary

An enzyme unwinds double-stranded RNA, but doesn't fully separate strands. This RNA unwinding activity permanently alters base pairing by converting adenosine to inosine, impacting gene silencing research.

More Related Videos

Glomerular Filtration
01:15

Glomerular Filtration

4.7K
Renal Corpuscle
01:20

Renal Corpuscle

6.9K

Related Experiment Videos

Last Updated: Jan 11, 2026

Nephrotic Syndrome I : Introduction
01:24

Nephrotic Syndrome I : Introduction

Published on: June 19, 2025

476
Glomerular Filtration
01:15

Glomerular Filtration

4.7K
Renal Corpuscle
01:20

Renal Corpuscle

6.9K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • RNA Biology

Background:

  • Double-stranded RNA (dsRNA) unwinding activities have been identified across various organisms.
  • Understanding the mechanisms and consequences of RNA unwinding is crucial for molecular biology.

Purpose of the Study:

  • To analyze the intermediates and products of a dsRNA unwinding reaction.
  • To elucidate the molecular changes and functional consequences of this RNA modification process.

Main Methods:

  • Analysis of RNA intermediates and final products using single-strand-specific ribonucleases.
  • Assessment of RNA rehybridization capabilities post-reaction.
  • Biochemical assays to identify base modifications in the reacted RNA.

Main Results:

  • The dsRNA unwinding reaction renders RNA sensitive to single-strand-specific ribonucleases but does not lead to complete strand separation.
  • Reacted RNA exhibits permanently altered base pairing properties, preventing rehybridization into the original duplex.
  • A significant conversion of adenosine residues to inosine residues was observed, proposed as the cause of irreversible base pairing changes.

Conclusions:

  • The RNA unwinding activity involves a covalent modification of adenosine to inosine.
  • This modification irreversibly alters RNA base pairing properties, with implications for biological functions.
  • The findings are relevant to understanding RNA-mediated gene regulation and antisense RNA technologies.
  • Meta_Description='Discover RNA unwinding activity: adenosine to inosine conversion alters base pairing, impacting antisense RNA research.'