Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nutritional and antioxidant changes in lentils and quinoa through fungal solid-state fermentation with Pleurotus ostreatus.

Bioresources and bioprocessing·2024

Same author

DVC-FISH to identify potentially pathogenic Legionella inside free-living amoebae from water sources.

Environmental research·2019

Same author

Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2016

Same author

Distribution and accumulation of Cd, Cu, Hg, Pb and Zn in the surface sediments of El Tobari Lagoon, central-East Gulf of California: An ecosystem associated with agriculture and aquaculture activities.

Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering·2013

Same author

Effect of a mixture of inulin and fructo-oligosaccharide on Lactobacillus and Bifidobacterium intestinal microbiota of patients receiving radiotherapy: a randomised, double-blind, placebo-controlled trial.

Nutricion hospitalaria·2013

Same author

In vivo study of the survival of Lactobacillus delbruecki subsp. bulgaricus CECT 4005T and Streptococcus thermophilus CECT 801 by DVC-FISH after consumption of fermented milk.

Journal of food science·2012

Search research articles

Related Experiment Video

Updated: Dec 29, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

IMD/ADM2_1-47, a factor that improves embryo quality.

J García-Hernández¹, B E Castro-Valenzuela¹, A Rodríguez-Borbón¹

¹Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua. Chihuahua, Chihuahua, Mexico.

|February 9, 2020

Summary

Supplementing bovine oocyte cultures with IMD/ADM2_1-47 peptide enhances embryo development. This peptide promotes oocyte competence, reduces apoptosis, and significantly increases blastocyst production rates for improved embryo quality.

Keywords:

Culture medium Cumulus-oocyte complexes Embryo production IMD/ADM2(1-47)Oocyte maturation

More Related Videos

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Published on: June 21, 2024

Human Egg Maturity Assessment and Its Clinical Application

Human Egg Maturity Assessment and Its Clinical Application

Published on: August 19, 2019

Related Experiment Videos

Last Updated: Dec 29, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Published on: June 21, 2024

Human Egg Maturity Assessment and Its Clinical Application

Human Egg Maturity Assessment and Its Clinical Application

Published on: August 19, 2019

Area of Science:

Reproductive Biology
Animal Science
Biotechnology

Background:

Oocyte competence is crucial for successful in vitro fertilization (IVF) and embryo development.
Current in vitro culture conditions aim to mimic the in vivo microenvironment for optimal oocyte maturation.
Improving oocyte competence and embryo quality remains a key challenge in assisted reproductive technologies.

Purpose of the Study:

To evaluate the efficacy of the IMD/ADM2_1-47 peptide in enhancing bovine oocyte competence and embryo quality.
To determine the optimal concentration of IMD/ADM2_1-47 for improving in vitro culture outcomes.
To assess the impact of IMD/ADM2_1-47 on oocyte apoptosis and blastocyst development.

Main Methods:

Bovine cumulus-oocyte complexes (COC) were cultured in vitro with varying concentrations of IMD/ADM2_1-47 peptide.
Oocyte competence was assessed by evaluating metaphase II (MII) stage progression and apoptosis markers (BAX/BCL-2 ratio, TUNEL assay).
Intracellular cyclic adenosine monophosphate (cAMPi) levels and blastocyst production rates were quantified.

Main Results:

Culture supplemented with 153 μg/mL of IMD/ADM2_1-47 resulted in a higher proportion of healthy MII oocytes.
A significant decrease in the BAX/BCL-2 mRNA ratio and reduced late apoptosis were observed in MII oocytes.
IMD/ADM2_1-47 maintained stable cAMPi levels and led to a two-fold increase in blastocyst production, with higher quality blastocysts (Grade I Stage 6 and Stage 5).

Conclusions:

The IMD/ADM2_1-47 peptide shows significant potential as a supplement for bovine oocyte in vitro maturation and fertilization.
This peptide improves oocyte quality by reducing apoptosis and enhancing developmental competence.
Supplementation with IMD/ADM2_1-47 notably boosts blastocyst formation rates and quality in bovine IVF.