Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

511
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
511
RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibiotic Resistance of <i>Acinetobacter</i> Isolated in a Spanish Veterinary Teaching Hospital.

Animals : an open access journal from MDPI·2026
Same author

A Sequence-Based Update on Amino Acid Substitutions in Influenza Polymerase Acidic Protein in Europe That Alter Baloxavir Susceptibility From 2009 to 2025.

Influenza and other respiratory viruses·2026
Same author

Obstructive sleep apnea risk and its association with diabetic foot ulcer in patients with type 2 diabetes.

Frontiers in endocrinology·2026
Same author

Baseline and follow-up association between adiposity and renal function: data from the ILERVAS cohort.

Obesity facts·2026
Same author

Food safety assessment of last mile delivery as an alternative food distribution system for ready-to-eat multi-ingredient salad bowls.

International journal of food microbiology·2026
Same author

Letter to the Editor: Discrepancy in the diagnosis criteria for gestational diabetes in 'Trends in the prevalence of gestational diabetes in Catalonia from 2010 to 2019'.

Primary care diabetes·2026

Related Experiment Video

Updated: Dec 29, 2025

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.0K

High-throughput sequencing and food microbiology.

Narciso M Quijada1, Marta Hernández2, David Rodríguez-Lázaro3

  • 1Laboratorio de Biología Molecular y Microbiología, Ibstituto tecnológico Agrario de Castilla y León (ITACyL), Valladolid, Spain.

Advances in Food and Nutrition Research
|February 10, 2020
PubMed
Summary

High-Throughput Sequencing (HTS) enables massive DNA sequencing, revolutionizing microbiology and food safety. This technology accelerates research in microbial diagnostics, genomics, and understanding foodborne infections.

Keywords:
Food microbiologyFood safetyHigh-throughput sequencingNext generation sequencingSequencingWhole genome sequencing

More Related Videos

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.4K
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
06:55

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

9.3K

Related Experiment Videos

Last Updated: Dec 29, 2025

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.0K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.4K
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
06:55

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

9.3K

Area of Science:

  • Microbiology
  • Food Safety
  • Bioinformatics
  • Genomics

Background:

  • Massive parallel sequencing, or High-Throughput Sequencing (HTS), generates millions to billions of short DNA reads.
  • HTS is transforming laboratory methods and computational analysis in microbiology and food safety.
  • Bioinformatics is essential for analyzing the vast datasets produced by HTS.

Purpose of the Study:

  • To highlight the impact of High-Throughput Sequencing on microbiology and food safety research.
  • To emphasize the role of bioinformatics in analyzing HTS data.
  • To showcase the advancements enabled by reduced time and cost in studying microbiota, microbiome, and metagenomes.

Main Methods:

  • Massive parallel sequencing (High-Throughput Sequencing) for DNA analysis.
  • Bioinformatics tools for computational analysis of large sequencing datasets.
  • Comparative analysis of microbiota, microbiome, and metagenome data.

Main Results:

  • Significant reduction in time and cost for microbiota, microbiome, and metagenome studies.
  • Accelerated progress in microbial diagnosis, taxonomy, and epidemiology.
  • Facilitated discovery of genes/variants and understanding of microbial associations with food spoilage and infections.

Conclusions:

  • High-Throughput Sequencing, coupled with bioinformatics, is a powerful tool for advancing microbiology and food safety.
  • The technology enables rapid progress in various research areas, from diagnostics to understanding foodborne pathogens.
  • HTS is crucial for the comprehensive study of microbial communities and their impact on food.