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Related Experiment Video

Updated: Dec 29, 2025

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis
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Profile analysis reveals endogenous RNAs regulate necrotizing enterocolitis progression.

Yan Xu1, Yiwen Liu1, Hanying Xie1

  • 1Department of Pediatrics, The Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu, 210004, China; The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002. China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|February 10, 2020
PubMed
Summary

Necrotizing enterocolitis (NEC) is a severe newborn condition. This study identifies differentially expressed long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) involved in NEC development, suggesting potential biomarkers for this gastrointestinal disease.

Keywords:
LncRNA-miRNA interaction networkLong non-coding RNAMicroRNANecrotizing enterocolitis

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Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Neonatology

Background:

  • Necrotizing enterocolitis (NEC) is a critical gastrointestinal disease affecting preterm newborns, with unclear underlying mechanisms.
  • Non-coding RNAs (ncRNAs) are implicated in intestinal diseases, but their specific roles in NEC pathogenesis are largely unknown.

Purpose of the Study:

  • To investigate the roles of long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in the development of NEC.
  • To identify potential molecular biomarkers and therapeutic targets for NEC.

Main Methods:

  • Differential expression analysis of lncRNAs, miRNAs, and mRNAs in a rat model of NEC.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.
  • Construction of a lncRNA-miRNA interaction network and validation of key lncRNAs using quantitative polymerase chain reaction (qPCR).

Main Results:

  • 1820 lncRNAs, 118 miRNAs, and 929 mRNAs were found to be differentially expressed in the NEC group.
  • Enriched pathways included apoptosis, autophagic cell death, TLR4, Notch, and mTOR signaling pathways.
  • Four novel differentially expressed lncRNAs were associated with the negative regulation of TLR4 and Notch signaling pathways.

Conclusions:

  • Differentially expressed lncRNAs, through interactions with miRNAs, may play a significant role in NEC pathogenesis.
  • These lncRNAs represent potential diagnostic biomarkers and therapeutic targets for necrotizing enterocolitis.