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Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
Published on: October 7, 2020
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Interplay between collagenase and undescended testes in Adamts16 knockout rats
Gulcan Sarila1, Tuya Bao2, Sanduni Amaya Abeydeera1
1Douglas Stephens Surgical Research Unit, Murdoch Children's Research Institute, Melbourne, Australia.
Journal of Pediatric Surgery
|February 11, 2020
Summary
Adamts16 enzyme deficiency in rats disrupts gubernacular migration, leading to undescended testes (UDT). This highlights Adamts16
Area of Science:
- Reproductive biology
- Developmental biology
- Biochemistry
Background:
- Testicular descent involves gubernaculum growth and extracellular matrix (ECM) remodeling.
- Adamts16, an ECM proteinase, is crucial for normal testicular descent.
- Adamts16 knockout rats exhibit cryptorchidism and infertility.
Purpose of the Study:
- Investigate the role of Adamts16 in congenital undescended testes (UDT).
- Examine the impact of Adamts16 deficiency on gubernacular migration during postnatal development in rats.
Main Methods:
- Histological analysis (H&E, Masson's trichrome) of wild-type and Adamts16 knockout rat tissues.
- Quantitative collagen fiber analysis in the inguinoscrotal fat pad (IFP).
Main Results:
- Gubernacular migration was significantly impaired in Adamts16 heterozygous and knockout rats.
- Increased collagen deposition around the gubernaculum was observed in Adamts16-deficient rats at day 8.
- Histological findings correlated with disrupted inguinoscrotal development.
Conclusions:
- Adamts16 deficiency causes gubernacular migration failure, resulting in UDT in rats.
- Adamts16 gene expression is critical for normal gubernacular migration.
- ECM remodeling, specifically collagen breakdown by Adamts16, is essential for testicular descent.
