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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Published on: September 2, 2021
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The rapidly advancing Class 2 CRISPR-Cas technologies: A customizable toolbox for molecular manipulations
Jingyi Wang1, Chenzi Zhang1,2, Bo Feng1,2,3
1Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
Journal of Cellular and Molecular Medicine
|February 11, 2020
Summary
CRISPR-Cas gene editing tools, derived from bacterial immunity, offer efficient, programmable DNA modification. Recent advances expand their applications in research, agriculture, and medicine.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- CRISPR-Cas systems are adaptive immune mechanisms in bacteria and archaea.
- These systems have been engineered into powerful gene-editing tools.
- Advancements in related fields accelerate CRISPR technology development.
Purpose of the Study:
- To summarize recent advances in CRISPR technologies and strategies.
- To highlight the diverse applications of CRISPR systems.
- To discuss the impact of CRISPR on genome editing and beyond.
Main Methods:
- Review of recent scientific literature on CRISPR-Cas systems.
- Analysis of technological advancements and engineering innovations.
- Compilation of reported applications across various disciplines.
Main Results:
- CRISPR-Cas tools are highly efficient, sequence-specific, programmable, and versatile.
- The CRISPR toolbox is rapidly expanding with new systems and functionalities.
- CRISPR is revolutionizing genome editing, transcriptional control, and genomic imaging.
Conclusions:
- CRISPR technologies have transformative potential in basic research.
- Significant agricultural and therapeutic applications are being realized.
- Continued innovation promises further expansion of CRISPR capabilities.
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