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Niastella caeni sp. nov., isolated from activated sludge
Mengyao Sheng1, Zhou Yang1, Xiaojun Yang2
1Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
International Journal of Systematic and Evolutionary Microbiology
|February 11, 2020
Summary
A novel bacterium, Niastella caeni HX-16-21^T, was identified from activated sludge. This new species degrades aromatic compounds and herbicides, expanding the known metabolic capabilities within the Niastella genus.
Area of Science:
- Microbiology
- Environmental Science
- Bacteriology
Background:
- Activated sludge harbors diverse microbial communities crucial for wastewater treatment.
- Understanding the metabolic capabilities of novel bacteria is essential for bioremediation and ecological studies.
- The genus Niastella comprises bacteria with known roles in various environmental processes.
Purpose of the Study:
- To isolate and characterize a novel bacterial strain from activated sludge.
- To determine the taxonomic and phylogenetic placement of the novel isolate.
- To investigate the metabolic potential of the new strain, particularly its degradation capabilities.
Main Methods:
- Isolation and cultivation of bacteria from activated sludge.
- Gram staining, morphology, and physiological tests for phenotypic characterization.
- 16S rRNA gene sequencing for phylogenetic analysis.
- Lipid, fatty acid, and respiratory quinone analysis.
- Genome sequencing and analysis (ANI, dDDH).
Main Results:
- A novel Gram-negative, aerobic, filamentous bacterium, designated HX-16-21^T, was isolated.
- Phylogenetic analysis placed HX-16-21^T within the genus Niastella, closely related to Niastella vici.
- The strain demonstrated the ability to degrade gentisate, protocatechuic acid, p-hydroxybenzoic acid, and herbicides quizalofop-p-ethyl and diclofop-methyl.
- Biochemical analyses revealed specific polar lipid, fatty acid, and menaquinone 7 (MK-7) profiles.
- Genomic data (8.1 Mb draft genome, 43.5% G+C content) and low ANI/dDDH values supported its novelty.
Conclusions:
- Strain HX-16-21^T represents a novel species within the genus Niastella, named Niastella caeni sp. nov.
- Niastella caeni exhibits significant biodegradation potential for aromatic compounds and specific herbicides.
- This discovery contributes to the understanding of microbial diversity and functional roles in activated sludge ecosystems.
