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Peptidoglycan Recognition Proteins (PGRPs) Modulates Mosquito Resistance to Fungal Entomopathogens in a Fungal-Strain

José L Ramirez1, Ephantus J Muturi1, Lina B Flor-Weiler1

  • 1Crop Bioprotection Research Unit, United States Department of Agriculture, National Center for Agricultural Utilization Research, Agricultural Research Service, Peoria, IL, United States.

Frontiers in Cellular and Infection Microbiology
|February 11, 2020
PubMed

Insights

Peptidoglycan recognition receptors (PGRPs) are crucial for mosquito immunity against fungal infections. Their depletion increases fungal growth and reduces mosquito survival, highlighting their role in disease vector control.

Area of Science:

  • Immunology
  • Entomology
  • Microbiology

Background:

  • Fungal entomopathogens pose a threat as mosquito vectors of human and animal diseases.
  • Effective fungal recognition by mosquitoes is vital for initiating an anti-fungal immune response.

Purpose of the Study:

  • To investigate the role of peptidoglycan recognition receptors (PGRPs) in mosquito resistance to fungal entomopathogens during early infection stages.

Main Methods:

  • Investigated the induction of specific PGRPs (PGRP-LA, -LB, -LD, -LE, -S1) upon fungal infection.
  • Analyzed temporal expression patterns of PGRPs post-infection.
  • Utilized RNAi silencing to deplete PGRPs and assess effects on fungal proliferation and mosquito survival.

Main Results:

  • Identified induction of PGRP-LA, -LB, -LD, -LE, and -S1 during infection with two fungal strains.
  • Observed significant PGRP upregulation at 60 hours post-infection, indicating temporal immune response.
  • RNAi-mediated depletion of PGRPs led to increased fungal proliferation and reduced mosquito survival, with strain-specific effects.

Conclusions:

  • PGRPs are integral to the mosquito's antifungal immune response.
  • These findings enhance understanding of host susceptibility factors influencing fungal entomopathogen infections in mosquitoes.
Keywords:
Aedes aegyptiPGRPantifungal immunityentomopathogenic fungimosquito immunity

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