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Updated: Dec 28, 2025

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues.

Elahe Masaeli^1,2, Christophe Marquette²

¹Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Frontiers in Bioengineering and Biotechnology

|February 11, 2020

Summary

This summary is machine-generated.

This study presents a novel hydrogel-free bioprinting method to create bilayer tissues. The technique uses a printed retina pigmented epithelium layer as living biopaper for fibroblast cell positioning, advancing tissue engineering.

Keywords:

cell layer inkjet bioprinting living biopaper tissue complexity tissue regeneration

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Area of Science:

Bioprinting and Tissue Engineering
Regenerative Medicine

Background:

Bioprinting is widely used for creating hydrogel-based 3D tissue models.
Existing methods often rely on hydrogels for tissue construction.

Purpose of the Study:

To introduce a unique direct-write bioprinting strategy for fabricating bilayer flat tissues.
To develop a hydrogel-free approach for constructing living tissue constructs.

Main Methods:

A direct-write bioprinting system was employed to fabricate a bilayer construct.
A retina pigmented epithelium (RPE) layer served as living biopaper for fibroblast positioning.
Inkjet droplet cell density was optimized for uniform cell layer formation.

Main Results:

A bilayer construct consisting of RPE and fibroblast layers was successfully fabricated without hydrogel.
Confocal imaging confirmed the formation of the bilayer structure.
The bioprinting system minimized shear stress, maintaining cell survival, though viability was slightly lower than controls over one week.

Conclusions:

The novel hydrogel-free direct-write bioprinting approach enables precise spatiotemporal positioning of cellular layers.
This technique offers an efficient method for developing living constructs, particularly for regenerating complex flat tissues.