Dyadobacter bucti sp. nov., isolated from subsurface sediment.

Area of Science:

• Microbiology
• Bacteriology
• Environmental Science

Background:

• The Qinghai-Tibetan Plateau harbors unique microbial ecosystems.
• Characterizing novel bacteria is crucial for understanding biodiversity and ecological roles.

Purpose of the Study:

• To isolate and characterize a novel bacterial species from a subsurface sediment sample.
• To determine the phylogenetic and genotypic relationship of the isolate to known bacteria.

Main Methods:

• Isolation and cultivation of bacteria from sediment samples.
• Gram staining, biochemical tests, and physiological characterization.
• 16S rRNA gene sequencing and whole-genome analysis.
• Phylogenetic analysis and determination of average nucleotide identity (ANI).

Main Results:

• A novel Gram-negative, yellow-pigmented, aerobic bacterium, strain QTA69T, was isolated.
• Phylogenetic analysis placed strain QTA69T within the genus Dyadobacter, closely related to D. sediminis, D. ginsengisoli, and D. psychrophilus.
• Physiological tests revealed optimal growth at 25-28°C, pH 7.0-8.0, and 0-1.0% NaCl.
• Genome sequencing showed a genome size of 8.41 Mbp with a G+C content of 46.87%.

Conclusions:

• Strain QTA69T represents a novel species, proposed as Dyadobacter bucti sp. nov.
• The study expands the known diversity of the Dyadobacter genus.
• Dyadobacter bucti is adapted to the specific environmental conditions of the Qinghai-Tibetan Plateau.