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HAADF-STEM image analysis for size-selected platinum nanoclusters.

Y Xia1,2, P Harrison1, I M Ornelas1

  • 1School of Physics and Astronomy, University of Birmingham, Birmingham, U.K.

Journal of Microscopy
|February 11, 2020
PubMed
Summary
This summary is machine-generated.

Precise measurement of platinum nanocluster size is crucial. Integrated HAADF intensity, extracted using a new ImageJ macro, proves more reliable than peak intensity or projected area for nanocluster size determination.

Keywords:
Image processingPt nanoclustersSTEM-HAADF

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Electron Microscopy

Background:

  • Accurate nanocluster size characterization is vital for understanding nanocluster-based materials.
  • Aberration-corrected scanning transmission electron microscopy (STEM) offers high-resolution imaging for direct size extraction.
  • Image analysis plays a critical role in obtaining reliable size data from STEM images.

Purpose of the Study:

  • To develop and validate a macro program for analyzing scanning transmission electron microscopy images of nanoclusters.
  • To investigate the influence of imaging parameters like magnification and objective lens defocus on nanocluster size measurements.
  • To identify the most robust metric for describing platinum nanocluster size from HAADF-STEM images.

Main Methods:

  • Utilized aberration-corrected scanning transmission electron microscopy (STEM) in high-angle annular dark field (HAADF) mode to image platinum nanoclusters.
  • Developed a macro program using existing ImageJ plug-ins for automated image analysis.
  • Extracted integrated HAADF intensity, peak intensity, and projected area of nanoclusters.

Main Results:

  • The developed macro program effectively assesses image intensity thresholds and filter blurring factors.
  • Integrated HAADF intensity was identified as a more robust descriptor of platinum nanocluster size compared to peak intensity and projected area.
  • The study quantified the effects of magnification and objective lens defocus on nanocluster size measurements.

Conclusions:

  • The developed ImageJ macro provides a reliable and accessible tool for analyzing HAADF-STEM images of nanoclusters.
  • Integrated HAADF intensity is recommended as the most robust parameter for accurate size determination of platinum nanoclusters.
  • The macro facilitates rapid assessment of factors impacting the accuracy of nanocluster size information extraction.