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Using photorespiratory oxygen response to analyse leaf mesophyll resistance.

Xinyou Yin1, Peter E L van der Putten2, Daniel Belay3

  • 1Centre for Crop Systems Analysis, Wageningen University & Research, P.O. Box 430, 6700 AK, Wageningen, The Netherlands. Xinyou.yin@wur.nl.

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Summary

A new method refines mesophyll conductance estimates by accounting for CO2 sources, improving accuracy in plants like tomato and rice. This approach better analyzes mesophyll resistance by considering photorespiration and respiration factors.

Keywords:
CO2 compensation pointCO2 transferInternal conductanceO2 responseRe-assimilationResistance

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Area of Science:

  • Plant Physiology
  • Photosynthesis Research
  • Biophysical Plant Science

Background:

  • Classical mesophyll conductance estimation methods overlook distinct CO2 resistance pathways from intercellular air spaces (IAS) and internal respiration (photorespiration, F, and dark respiration, Rd).
  • This oversight leads to apparent dependencies of mesophyll conductance on the ratio of respiration to net photosynthesis ((F + Rd)/A), influenced by complex cellular anatomy.

Purpose of the Study:

  • To develop and validate a novel method for estimating mesophyll conductance that incorporates the parameter 'm', representing key anatomical properties.
  • To assess the impact of this new method on understanding CO2 refixation within different plant species under varying conditions.

Main Methods:

  • A new parameter 'm' (0 ≤ m ≤ 1) was introduced to lump anatomical properties influencing CO2 diffusion.
  • Gas exchange and chlorophyll fluorescence measurements were employed across varying oxygen levels in tomato and rice leaves.
  • The method was applied to datasets where the (F + Rd)/A ratio varied to estimate 'm' and subsequently mesophyll conductance.

Main Results:

  • The parameter 'm' was estimated as 0.3 for tomato and 0.0 for rice, indicating classical methods are suitable for rice.
  • Mesophyll conductance, adjusted for 'm', showed responses to irradiance, CO2, and O2 levels, mirroring stomatal conductance patterns.
  • Differences in 'm' values resulted in varied internal CO2 refixation fractions between tomato and rice, though total refixation remained similar.

Conclusions:

  • The developed method effectively analyzes mesophyll resistance by accounting for internal CO2 sources.
  • The parameter 'm' provides a simplified yet effective way to represent crucial leaf anatomical features.
  • Findings align with CO2 compensation point estimates, validating the improved approach to mesophyll conductance analysis.