Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

21.3K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
21.3K
Transgenic Plants02:50

Transgenic Plants

8.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.3K
Plant Tissue Culture02:57

Plant Tissue Culture

40.0K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
40.0K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.4K
Overview
79.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PII interactions with the acetyl-CoA carboxylase subunits BADC and BCCP co-regulate lipid and nitrogen metabolism in Arabidopsis.

Plant physiology·2025
Same author

Complete replacement of Arabidopsis oil-producing enzymes with heterologous diacylglycerol acyltransferases.

Plant physiology·2025
Same author

Identification and Characterization of Lipid Droplet-Associated Protein (LDAP) Isoforms from Tung Tree (<i>Vernicia fordii</i>).

Plants (Basel, Switzerland)·2025
Same author

Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.

Plant physiology·2024
Same author

The first intron and promoter of Arabidopsis DIACYLGLYCEROL ACYLTRANSFERASE 1 exert synergistic effects on pollen and embryo lipid accumulation.

The New phytologist·2024
Same author

Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils.

Nature communications·2024

Related Experiment Video

Updated: Dec 28, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.1K

Gene editing in plants: assessing the variables through a simplified case study.

Jay Shockey1

  • 1Agricultural Research Service, Southern Regional Research Center, Commodity Utilization Research Unit, United States Department of Agriculture, 1100 Robert E. Lee Blvd., New Orleans, LA, 70124, USA. Jay.Shockey@usda.gov.

Plant Molecular Biology
|February 11, 2020
PubMed
Summary

Optimizing plant genome editing involves understanding CRISPR construct design. This study in Arabidopsis thaliana reveals how promoter choice and target site characteristics influence editing success and unintended mutations.

Keywords:
ArabidopsisCRISPRCas9Gene editingHydroxy fatty acidsSingle-guide RNA

More Related Videos

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives
06:57

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

2.1K
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
07:28

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Published on: October 4, 2021

3.8K

Related Experiment Videos

Last Updated: Dec 28, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.1K
Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives
06:57

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

2.1K
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
07:28

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Published on: October 4, 2021

3.8K

Area of Science:

  • Plant molecular biology
  • Genetics and genomics
  • Biotechnology

Background:

  • CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has transformed gene editing.
  • Efficient and heritable genome editing in plants is crucial for research and agriculture.
  • CRISPR construct design significantly impacts editing outcomes.

Purpose of the Study:

  • To investigate variables affecting successful heritable plant genome editing.
  • To compare the efficacy of different CRISPR construct designs in Arabidopsis thaliana.
  • To assess the impact of promoter strength and target site characteristics on editing efficiency.

Main Methods:

  • Utilized CRISPR-Cas9 technology in Arabidopsis thaliana.
  • Compared CRISPR constructs with varying promoters.
  • Analyzed editing efficiency at target sites with different guanine-cytosine content.
  • Observed nuclease activity with imperfectly matched single guide RNAs.

Main Results:

  • Different promoters and target site characteristics influence editing levels.
  • Editing efficiency varies across different genomic locations within a gene.
  • Off-target mutations can occur even with imperfectly matched single guide RNAs.

Conclusions:

  • Successful heritable plant genome editing depends on multiple factors, including construct design.
  • Careful monitoring for unintended mutations is essential alongside optimizing for robust editing.
  • Further research can refine CRISPR strategies for precise plant genome modifications.