Modified Potentiometric Screen-Printed Electrodes Based on Imprinting Character for Sodium Deoxycholate...

Area of Science:

• Electrochemistry
• Materials Science
• Analytical Chemistry

Background:

• Potentiometric sensors are crucial for compound determination in various matrices.
• Bile acid salts, like sodium deoxycholate (NaDC), require efficient and novel sensing methods.
• Molecular imprinting technology offers a pathway for creating selective recognition materials.

Purpose of the Study:

• To develop and characterize novel potentiometric sensors for the sensitive and selective detection of sodium deoxycholate (NaDC).
• To evaluate the performance of NaDC-imprinted polymer (MIP) based sensors for bile acid salt determination.
• To explore the application of these sensors in analyzing biological samples.

Main Methods:

• Synthesis of NaDC-imprinted polymer (MIP) beads using acrylamide, EGDMA, NaDC, and BPO via thermal polymerization.
• Fabrication of sensors on a screen-printed platform with MIP beads and single-walled carbon nanotubes (SWCNTs) as an ion-to-electron transducer.
• Characterization of MIPs using Fourier Transform-Infrared (FT-IR) spectroscopy and Scanning Electron Microscopy (SEM).

Main Results:

• The developed sensors exhibited high sensitivity with a near-Nernestian slope (-60.1 ± 0.9 mV/decade) and a limit of detection of 4.7 × 10^-5 M.
• The sensors demonstrated excellent selectivity, long operational lifetime, and high potential stability.
• The ion-to-electron transduction using SWCNTs ensured reproducible and accurate results over extended periods.
• Successful determination of human serum albumin (HSA) in human serum samples was achieved, indicating potential for biological applications.

Conclusions:

• The novel NaDC-MIP based potentiometric sensors provide a highly sensitive, selective, and stable platform for bile acid salt detection.
• The integration of SWCNTs enhances sensor performance, ensuring reliable measurements.
• These sensors hold promise for accurate determination of analytes in complex biological matrices like human serum.