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Related Experiment Video

Updated: Dec 28, 2025

Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis

Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis

Published on: December 18, 2010

Cathelicidin preserves intestinal barrier function in polymicrobial sepsis.

Jeffery Ho¹, Hung Chan¹, Yonghao Liang¹

¹Department of Anaesthesia and Intensive Care and Peter Hung Pain Research Institute, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region, China.

Critical Care (London, England)

|February 12, 2020

Summary

Cathelicidin (Cnlp) is crucial for maintaining intestinal barrier integrity during sepsis. Vitamin D3

Keywords:

Antimicrobial peptide Bacterial translocation LL-37 Sepsis

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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

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Published on: June 15, 2019

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Published on: December 9, 2022

Area of Science:

Immunology
Gastroenterology
Microbiology

Background:

The intestinal epithelium forms a critical barrier between the gut microbiota and the bloodstream.
Sepsis is associated with impaired intestinal barrier function, exacerbating systemic inflammation.
Cathelicidin (Cnlp) expression is altered in sepsis, but its role in intestinal barrier dysfunction remains unclear.

Purpose of the Study:

To investigate the role of cathelicidin (Cnlp) in polymicrobial sepsis-induced intestinal barrier dysfunction.
To evaluate the therapeutic potential of vitamin D3 in a murine model of polymicrobial sepsis.

Main Methods:

Cecal-ligation and puncture (CLP) model in wild-type and Cnlp knockout mice.
Assessment of mortality, sepsis scores, ileal histology, biochemistry, immunology, and transcriptomics.
Evaluation of prophylactic and therapeutic administration of vitamin D3 (cholecalciferol and 1alpha, 25-dihydroxyvitamin D3).

Main Results:

Cnlp knockout mice exhibited increased mortality, sepsis severity, intestinal permeability, and bacterial load post-CLP.
Cnlp deficiency led to goblet cell loss, increased apoptosis, and altered immune cell infiltration (M1 macrophages, neutrophils).
Treatment with 1alpha, 25-dihydroxyvitamin D3 reduced mortality and sepsis severity, while cholecalciferol administration worsened outcomes.

Conclusions:

Endogenous cathelicidin (Cnlp) is essential for preserving intestinal barrier integrity during polymicrobial sepsis.
Cnlp modulates immune cell infiltration, contributing to barrier defense.
1alpha, 25-dihydroxyvitamin D3 shows therapeutic potential for sepsis, whereas cholecalciferol may be detrimental.