Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell to cell interactions and normal mammary gland function.

S Z Haslam1

  • 1Department of Anatomy, Michigan State University, East Lansing 48824.

Journal of Dairy Science
|October 1, 1988
PubMed
Summary

Mammary fibroblasts influence estrogen response in epithelial cells through substratum effects and direct contact, impacting progesterone receptor levels and DNA synthesis. These interactions suggest complex in vivo regulatory roles for stromal cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pubertal exposure to high fat diet causes mouse strain-dependent alterations in mammary gland development and estrogen responsiveness.

International journal of obesity (2005)·2010
Same author

Extracellular matrix, Rac1 signaling, and estrogen-induced proliferation in MCF-7 breast cancer cells.

Breast cancer research and treatment·2007
Same author

Reciprocal regulation of extracellular matrix proteins and ovarian steroid activity in the mammary gland.

Breast cancer research : BCR·2001
Same author

Fibronectin and the alpha(5)beta(1) integrin are under developmental and ovarian steroid regulation in the normal mouse mammary gland.

Endocrinology·2001
Same author

Estrogen and estrogen plus progestin act directly on the mammary gland to increase proliferation in a postmenopausal mouse model.

Journal of cellular physiology·2001
Same author

Proliferative effects of combination estrogen and progesterone replacement therapy on the normal postmenopausal mammary gland in a murine model.

American journal of obstetrics and gynecology·2001

Area of Science:

  • Cell Biology
  • Endocrinology
  • Histology

Background:

  • The mammary gland comprises epithelial and nonepithelial cells.
  • Cell-cell interactions are crucial for mammary gland function.
  • Estrogen regulates mammary gland epithelial cell activity.

Purpose of the Study:

  • To investigate the regulatory role of mammary stromal fibroblasts in epithelial cell responses.
  • To examine how fibroblast-epithelial interactions influence estrogenic regulation.
  • To analyze the impact on progesterone receptor concentration and DNA synthesis.

Main Methods:

  • In vitro cell culture system using mammary epithelial and fibroblast cells.
  • Co-culture models to study cell-cell interactions.
  • Assays to measure progesterone receptor concentration and DNA synthesis.

Related Experiment Videos

Main Results:

  • Mammary fibroblasts modulate estrogen responsiveness in epithelial cells via two distinct mechanisms.
  • Fibroblasts promote estrogen-dependent progesterone receptor increases through a substratum effect (collagen type I).
  • Fibroblast-mediated estrogen-dependent epithelial proliferation requires metabolic activity and close contact.
  • Estrogen stimulates fibroblast DNA synthesis, indicating bidirectional signaling.

Conclusions:

  • Mammary fibroblasts play a significant regulatory role in epithelial cell function.
  • Interactions between fibroblasts and epithelial cells influence estrogenic pathways.
  • These in vitro findings suggest potential in vivo mechanisms governing mammary gland responsiveness.