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Updated: Dec 28, 2025
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Simultaneous multiplane imaging with reverberation two-photon microscopy
Devin R Beaulieu1, Ian G Davison2,3, Kıvılcım Kılıç4
1Department of Electrical Engineering, Boston University, Boston, MA, USA. devinrbeaulieu@icloud.com.
Abstract:
Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.
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