Biological and physiological characteristics of human cumulus cells in adherent culture condition
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Published on: November 28, 2019
Somayyeh Sadat Tahajjodi1,2,3, Ehsan Farashahi Yazd1,3, Azam Agha-Rahimi2,3
1Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Background:
Cumulus cells, as oocyte nurse cells, provide a suitable microenvironment with growth factors and cellular interactions required for oocyte maturation. Thus, these cells may serve as a natural niche for in vitro studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.
Objective:
The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.
Materials And Methods:
In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including FOXL2, CYP19A1, FSHR, AMHR, and LHR. The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.
Results:
In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.
Conclusion:
Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for in vitro maturation (IVM) and in vitro gametogenesis (IVG) studies.
