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Parallel single-cell optical transit dielectrophoresis cytometer.

Azita Fazelkhah1, Samaneh Afshar1, Nicholas Durham2

  • 1Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, Canada.

Electrophoresis
|February 12, 2020
PubMed
Summary

We developed an optical transit dielectrophoresis (DEP) flow cytometer for high-throughput, label-free single-cell analysis. This system measures cell dielectric properties by analyzing velocity changes in microfluidic channels, achieving rapid parallel analysis.

Keywords:
CHO cellsDEPDielectric propertiesMicrofluidicSingle-cell

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Area of Science:

  • Biophysics
  • Microfluidics
  • Analytical Chemistry

Background:

  • Dielectrophoresis (DEP) is a powerful technique for manipulating and analyzing cells based on their dielectric properties.
  • Existing methods for single-cell dielectric analysis can be limited in throughput and require complex sample preparation.
  • High-throughput, label-free analysis is crucial for advancing cell biology and diagnostics.

Purpose of the Study:

  • To develop and validate an optical transit dielectrophoresis (DEP) flow cytometer for parallel single-cell analysis.
  • To enable label-free, quantitative measurement of cell dielectric properties at high throughput.
  • To demonstrate the system's capability for analyzing biological cells.

Main Methods:

  • Utilized dielectrophoresis (DEP) actuation in a microfluidic channel to perturb cell velocity.
  • Employed dual LED light sources and a linear optical array detector to measure cell transit velocity.
  • Designed a wide microfluidic channel (∼18 mm) for simultaneous analysis of over 200 cells.
  • Achieved analysis rates exceeding 250 10 µm spheres per second.

Main Results:

  • Demonstrated parallel single-cell analysis with DEP-induced velocity perturbations.
  • Quantitatively measured the dielectric response (Clausius-Mossotti factor) of viable CHO cells from 100 kHz to 6 MHz.
  • Validated results against previously reported measurements, confirming accuracy.
  • Achieved rapid analysis initiation (within 10 s) after sample introduction.

Conclusions:

  • The optical transit DEP flow cytometer provides a simple, modular, and high-throughput platform for label-free single-cell dielectric analysis.
  • The system offers quantitative dielectric measurements, enabling detailed cell characterization.
  • This technology has significant potential for applications in cell biology, drug discovery, and diagnostics.