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Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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TUTORIAL: ION ACTIVATION IN TANDEM MASS SPECTROMETRY USING ULTRA-HIGH RESOLUTION INSTRUMENTATION.

Parisa Bayat1, Denis Lesage1, Richard B Cole1

  • 1Faculté des Sciences et Ingénierie, Sorbonne Université, IPCM (UMR 8232), F-75252, Paris, France.

Mass Spectrometry Reviews
|February 12, 2020
PubMed
Summary
This summary is machine-generated.

This tutorial overviews ion activation techniques in tandem mass spectrometry, explaining theories behind collision-, photon-, and electron-mediated methods for fragmenting ions to reveal molecular structures.

Keywords:
collision-induced dissociationelectron-induced dissociationphoton-induced dissociationtandem mass spectrometryunimolecular dissociation

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Biochemistry

Background:

  • Tandem mass spectrometry (MS/MS) is crucial for structural elucidation.
  • Ion activation techniques are key steps in MS/MS for inducing dissociation.
  • These techniques are vital in chemistry and biology for analyzing molecular structures and properties.

Purpose of the Study:

  • To provide a tutorial overview of ion activation theories in tandem mass spectrometry.
  • To discuss the fundamental principles of various ion activation techniques.
  • To contextualize these techniques within ultra-high resolution mass spectrometry.

Main Methods:

  • Collision-Induced Dissociation (CID)
  • Photon-Induced Dissociation (PID)
  • Electron-Induced Dissociation (EID)
  • Other techniques: PQD, HCD, SORI, SID, BIRD, IRMPD, UVPD, EPD, ECD, EDD, ETD

Main Results:

  • Detailed explanation of unimolecular dissociation theories.
  • Discussion of diverse ion activation methods including CID, PID, and EID.
  • Contextualization of techniques for modern ultra-high resolution mass spectrometers.

Conclusions:

  • Ion activation is fundamental to tandem mass spectrometry.
  • A variety of techniques exist, each with specific applications.
  • Understanding these methods is essential for advanced structural analysis.