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Circular RNA expression profiles and bioinformatic analysis in coronary heart disease

Fangpu Yu1, Yuanyuan Tie1, Ya Zhang1

  • 1The Key Laboratory of Cardiovascular Remodeling & Function Research, Chinese Ministry of Education, Chinese Ministry of Health & Chinese Academy of Medical Sciences, & The State & Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong, PR China.

Epigenomics
|February 12, 2020
PubMed

Insights

Circular RNAs (circRNAs) show altered expression in coronary heart disease (CHD). These dysregulated circRNAs may influence CHD by acting as competing endogenous RNAs (ceRNAs).

Area of Science:

  • Genomics
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Coronary heart disease (CHD) is a significant global health concern.
  • The role of circular RNAs (circRNAs) in CHD pathogenesis remains largely unexplored.

Purpose of the Study:

  • To investigate the expression profile of circRNAs in patients with CHD.
  • To elucidate the potential role of circRNAs in the pathophysiology of CHD.

Main Methods:

  • CircRNA expression profiling was performed using sequence analysis on peripheral blood mononuclear cells from 70 CHD patients and 30 controls.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate selected circRNAs in human atherosclerotic coronary arteries.

Main Results:

  • A total of 2283 downregulated and 85 upregulated circRNAs were identified in CHD patients.
  • Parental genes of the top 100 dysregulated circRNAs are associated with metabolism and protein modification.
  • Twelve circRNAs were predicted to upregulate their CHD-related parental genes via miRNA sponge mechanisms.
  • Six of eight validated circRNAs in atherosclerotic coronary arteries showed results consistent with peripheral blood mononuclear cell sequencing.

Conclusions:

  • Dysregulated circRNAs exhibit altered expression patterns in CHD.
  • circRNAs may function as competing endogenous RNAs (ceRNAs) in CHD.
  • These findings suggest a potential role for circRNAs in the development and progression of CHD.

Keywords:
RNA sequencing (RNA-seq)circular RNAs (circRNAs)coronary heart disease (CHD)miRNA

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