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Published on: November 10, 2008
Novel ELISAs to measure total and phosphorylated tau in cerebrospinal fluid
Takeshi Kawarabayashi1, Takumi Nakamura2, Kazuya Miyashita3
1Department of Neurology, Dementia Research Center, Geriatrics Research Institute and Hospital, 3-26-8 Otomo-machi, Maebashi, 371-0847, Japan; Department of Neurology, Gunma University Hospital, 3-39-15 Showamachi, Maebashi, 371-8511, Japan; Department of Social Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8216, Japan.
Abstract:
Cerebrospinal fluid (CSF) total tau (t-tau) and tau protein phosphorylated at threonine 181 (p181tau) are established biomarkers for Alzheimer's disease (AD). Herein, we measured t-tau and p181tau to evaluate novel enzyme-linked immunosorbent assays (ELISAs) using 72 CSF samples including from patients with AD with dementia (ADD) and various neurodegenerative diseases. Our assay system showed good correlations with widely used ELISA systems for t-tau and p181tau and showed that serum and hemoglobin contamination in CSF samples did not decrease sensitivity. Significant increases in both t-tau and p181tau levels were observed in ADD. These findings suggested that our ELISAs were reliable assays for CSF t-tau and p181tau similar to commonly used ELISAs.
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