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Updated: Dec 28, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Genetic variation at the interleukin-18 locus is associated with wheat-dependent exercise-induced anaphylaxis in the
Xiang Gao1, Liping Wen1, Hong Li1
1Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Beijing Key Laboratory of Precision Medicine for Diagnosis and Treatment of Allergic Diseases, Beijing, China.
Objective:
To investigate the association of single nucleotide polymorphisms (SNPs) at the interleukin (IL)-18 locus with wheat-dependent exercise-induced anaphylaxis (WDEIA) in the Han Chinese population.
Method:
130 patients with WDEIA and 600 healthy subjects were recruited for this study. Three tag SNPs (rs360729, rs360717 and rs1946518) were selected and genotyped, and total IgE and specific IgE levels were measured using the ImmunoCAP system.
Results:
After Bonferroni correction, the frequency of the G-allele in rs1946518 in the WDEIA group was significantly higher than that in control group (P = 0.0015). Genotypic distributions of rs1946518 significantly differed between WDEIA and control groups, and compared with the TT genotype, the homozygote GG genotype was associated with a higher risk of WDEIA (P = 0.0031). At position rs1946518, the log-transformed total IgE values were significantly higher in the group with the heterozygous GT genotype than in TT genotype group (P = 0.0024). Haplotype AGG formed by three SNPs alleles occurred significantly more frequently in WDEIA group than in control group (P = 0.003).
Conclusion:
The minor allele G at position rs1946518 might serve as a marker for the risk of WDEIA.
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