Enhanced removal of bisphenol A from contaminated soil by coupling Bacillus subtilis HV-3 with electrochemical system

Exposure to endocrine disruptors interferes with the synthesis, release, transport and metabolic activities of hormones, thus impairing human health significantly. Bisphenol A (BpA), an endocrine disruptor, commonly released into the environment by industrial activities and needs immediate attention. This study aims at investigating the process and prospects of deploying bio-electrochemical systems (BES) for the removal of BpA from artificially contaminated soil using Bacillus subtilis HV-3. The BES was setup with desired operating conditions: initial concentration of BpA (80-150 mg/L), pH (3-11) and applied potential voltage (0.6-1.4 V). Under optimized conditions (initial BpA concentration, 100 mg/L; pH 7; and applied voltage 1.0 V), close to 98% degradation of BpA was achieved. The intermediates produced during degradation were analysed using High performance liquid chromatography-Mass spectrometry and the possible degradation pathway was elucidated. Phytotoxicity studies in the remediated soil with Phaseolus mungo confirmed the environmental applicability of the BES system.