Related Experiment Video
Updated: Dec 28, 2025
Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus
Published on: May 24, 2020
Assessment of Culex pipiens bioforms in the world's southernmost distribution limit
María Victoria Cardo1,2, Alejandra Rubio1,2, Darío Vezzani2,3
1Universidad Nacional de San Martin-Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Investigación e Ingeniería Ambiental, Ecología de Enfermedades Transmitidas por Vectores (2eTV), San Martín, Provincia de Buenos Aires, Argentina.
Abstract:
The mosquito Culex pipiens s.s. L. occurs as two bioforms that differ in physiology and behaviour affecting virus transmission cycles. To assess the occurrence of Cx. pipiens bioforms in the southernmost limit of its distribution, specimens were collected aboveground in southern Buenos Aires Province and east Patagonia, Argentina. Ten larvae and 25 adults were individually processed and identified by polymerase chain reaction (PCR) amplification of Ace-2 and CQ11 loci. Culex quinquefasciatus Say (one larva, two adults), Cx. pipiens f. molestus (one larva, one adult) and one adult of hybrid origin were identified in Buenos Aires Province; only Cx. pipiens f. molestus was recorded in Patagonia (eight larvae, 21 adults). The potential absence of bioform pipiens and its implications in arbovirus enzootic cycles is discussed.
