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LncRNA TTN-AS1 acts as sponge for miR-15b-5p to regulate FBXW7 expression in ovarian cancer

Sheng Miao1, Jia Wang1, Lili Xuan1

  • 1Department of Gynaecology and Obstetrics, China-Japan Union Hospital of Jilin University, Changchun, China.

Biofactors (Oxford, England)
|February 13, 2020
PubMed

Insights

Titin-antisense RNA1 (TTN-AS1) acts as a tumor suppressor in ovarian cancer by inhibiting cell proliferation and promoting apoptosis. This long noncoding RNA regulates the miR-15b-5p/FBXW7 axis, offering potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in cancer progression.
  • The role of Titin-antisense RNA1 (TTN-AS1) in ovarian cancer was previously unknown.

Purpose of the Study:

  • To investigate the function and mechanism of TTN-AS1 in ovarian cancer.
  • To explore the potential regulatory axis involving TTN-AS1, miR-15b-5p, and FBXW7.

Main Methods:

  • Quantitative reverse-transcription PCR to measure gene expression.
  • Bioinformatic prediction and luciferase reporter assays to validate interactions.
  • Cell proliferation, colony formation, and apoptosis assays to assess functional effects.

Main Results:

  • TTN-AS1 expression was decreased in ovarian cancer tissues and cells.
  • TTN-AS1, miR-15b-5p, and FBXW7 were validated as interacting components.
  • Overexpression of TTN-AS1 suppressed ovarian cancer cell proliferation and colony formation while promoting apoptosis.

Conclusions:

  • The TTN-AS1/miR-15b-5p/FBXW7 axis plays a significant role in ovarian cancer.
  • TTN-AS1 functions as a tumor suppressor in ovarian cancer.
  • This axis may serve as a potential therapeutic biomarker for ovarian cancer treatment.
Keywords:
FBXW7TTN-AS1miR-15b-5povarian cancer

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