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Published on: July 10, 2018
C1q/TNF-related protein-9 alleviates airway inflammation in asthma
Min Qian1, Qingxiao Yang2, Jindong Li3
1Department, of Neonatology, The Second Hospital of Jilin University, Changchun City 130041, PR China.
Insights
C1q tumor necrosis factor-related protein 9 (CTRP9) levels are reduced in childhood asthma. CTRP9 demonstrates anti-inflammatory effects, suggesting its potential as a therapeutic target for asthma management.
Area of Science:
- Immunology
- Respiratory Medicine
- Biochemistry
Background:
- Asthma is a chronic inflammatory respiratory disorder often beginning in childhood.
- Novel therapeutic targets for childhood asthma are crucial due to increasing prevalence.
- C1q tumor necrosis factor-related protein 9 (CTRP9) is a recently identified anti-inflammatory factor.
Purpose of the Study:
- To investigate the effects of CTRP9 on asthma.
- To evaluate CTRP9 as a potential therapeutic target for childhood asthma.
Main Methods:
- Measured CTRP9 mRNA and protein levels in asthmatic children and healthy controls.
- Assessed CTRP9's anti-inflammatory effects in vitro using peripheral blood mononuclear cells (PBMCs) and sputum cells.
- Evaluated CTRP9's therapeutic potential in a mouse model of asthma.
Main Results:
- CTRP9 mRNA and protein levels were significantly lower in asthmatic children compared to healthy controls.
- CTRP9 expression negatively correlated with pro-inflammatory cytokines TNF-α, IL-1β, and IL-6.
- CTRP9 suppressed pro-inflammatory factors in vitro and alleviated asthma symptoms in a mouse model.
Conclusions:
- CTRP9 exhibits anti-inflammatory properties relevant to asthma.
- CTRP9 may serve as a potential therapeutic agent for alleviating airway inflammation and remodeling in asthma.
Backgrounds:
Asthma is characterized as inflammatory disorder in the respiratory system with increasing tendency. Most of the asthma patients suffered from the disease since childhood. Thus, developing novel therapeutic targets of childhood asthma is necessary. Here, we conducted the present study to investigate the effects of CTRP9 (C1q tumor necrosis factor-related protein 9), a newly identified anti-inflammatory factor, on asthma.
Methods:
Sixty asthmatic children (30 moderate and 30 mild) were recruited. The mRNA level of CTRP9 in peripheral blood mononuclear cells (PBMCs) and protein level of CTRP9 in serum and induced sputum (IS) samples from asthma patients and healthy controls (HCs) were measured by qPCR and ELISA, respectively. The anti-inflammatory effects of CTRP9 was determined in vitro and potential therapeutic effect on asthma was evaluated in mouse model.
Results:
The mRNA and protein levels of CTRP9 was significantly down-regulated in asthmatics than HCs. Furthermore, the expression level of CTRP9 was negatively correlated with the expression of TNF-α, IL-1β, and IL-6 in PBMCs. The CTRP9 significantly suppressed the expression of pro-inflammatory factors in PBMCs and sputum cells from asthma patients in vitro. And delivering CTRP9 into mouse model of asthma showed disease alleviation.
Conclusion:
Our data here indicated that CTRP9 may alleviate airway inflammation and remodeling in asthma.
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