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In Vitro Hypocholesterolemic Effect of Coffee Compounds
Filipe Manuel Coreta-Gomes1,2, Guido R Lopes1, Cláudia P Passos1
1LAQV-REQUIMTE, Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Nutrients
|February 14, 2020
Summary
Coffee
Area of Science:
- Food Science
- Nutritional Biochemistry
- Analytical Chemistry
Background:
- Cholesterol bioaccessibility influences absorption and hypocholesterolemic potential.
- Investigating coffee's impact on cholesterol solubility is crucial for dietary interventions.
- An in vitro model using bile salts assessed cholesterol bioaccessibility.
Purpose of the Study:
- To determine the effect of espresso coffee and its extracts on cholesterol solubility.
- To elucidate the mechanisms by which coffee components influence cholesterol bioaccessibility.
- To quantify the contribution of coffee polysaccharides and lipids to cholesterol solubility.
Main Methods:
- Purification and characterization of coffee polysaccharide extracts (arabinogalactans, galactomannans).
- Lipid extraction from coffee samples.
- Quantitative 13C NMR to measure 13C-4 cholesterol solubility in bile salt micelles.
Main Results:
- Coffee polysaccharides (arabinogalactans, galactomannans) sequestered bile salts, reducing cholesterol solubility.
- Coffee lipids decreased cholesterol solubility via co-solubilization in bile salt micelles.
- Both components exhibited an additive effect on cholesterol bioaccessibility.
Conclusions:
- Coffee soluble fiber significantly reduces cholesterol bioaccessibility by binding bile salts.
- Coffee lipids contribute to reduced cholesterol bioaccessibility through a different mechanism.
- These findings are vital for formulating hypocholesterolemic food ingredients.
Keywords:
NMRbile saltsbioaccessibilitycholesterolcoffeehypocholesterolemic effectlipidpolysaccharidessequestrationMore Related Videos
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