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Related Experiment Videos

Cell surface oligosaccharide modulation during differentiation: III. Lectin affinity class distributions.

P L Mann1, C M Swartz, D T Holmes

  • 1Department of Anatomy, University of New Mexico, School of Medicine, Albuquerque 87131.

Mechanisms of Ageing and Development
|July 1, 1988
PubMed
Summary
This summary is machine-generated.

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Cellular senescence involves cell surface changes, including decreased mannosyl, galactosyl, and N-acetyl-glucosamine residues. These changes are linked to altered lectin binding affinities, potentially impacting cell signaling.

Area of Science:

  • Cell Biology
  • Biochemistry

Background:

  • Cellular senescence is preceded by complex cell surface alterations.
  • Previous studies identified quantitative decreases in cell surface mannosyl, galactosyl, and N-acetyl-glucosamine residues.
  • These changes correlate with altered ligand/lectin membrane mobility, suggesting functional significance.

Purpose of the Study:

  • To investigate the biological significance of cell surface changes during senescence.
  • To analyze lectin binding events using a synthetic ligand competition assay.

Main Methods:

  • Development of a synthetic ligand competition assay.
  • Analysis of lectin binding events and affinity class distributions.

Main Results:

  • The number of binding affinity class distributions is highly restricted.

Related Experiment Videos

  • PDL-dependent mannosyl changes result from the loss of high-affinity binding sites.
  • PDL-dependent changes in galactosyl and N-acetyl glucosamine binding are due to altered affinity class distributions.

    • Conclusions:

    • Cell surface lectin binding changes during senescence are characterized by restricted affinity distributions.
    • These alterations in binding affinity may provide a basis for signal transduction at the cell surface.