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Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Metastasis via the miR-155-5p/FGF7 Axis and Predicts
Hong-Wu Ma1, Da-Yong Xi2, Jian-Zhong Ma3
1Department of Emergency Medicine, Lanzhou University Second Hospital, Lanzhou, Gansu, China.
Disease Markers
|February 14, 2020
Summary
Actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) promotes gastric cancer (GC) progression by regulating the miR-155-5p/FGF7 axis. This oncogenic lncRNA may serve as a potential therapeutic target for GC patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) is implicated in various cancers.
- Its role in gastric cancer (GC) progression remained largely uncharacterized.
Purpose of the Study:
- To investigate the biological function and mechanism of AFAP1-AS1 in gastric cancer progression.
- To explore the potential of AFAP1-AS1 as a therapeutic target for GC.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for AFAP1-AS1 expression analysis.
- Loss-of-function assays to assess AFAP1-AS1's impact on GC cell behavior.
- Bioinformatics, luciferase reporter, and RNA immunoprecipitation (RIP) assays to identify molecular interactions.
- Rescue experiments to validate the AFAP1-AS1-miR-155-5p-FGF7 axis.
Main Results:
- AFAP1-AS1 expression was significantly upregulated in GC tissues and cell lines, correlating with poor prognosis.
- Knockdown of AFAP1-AS1 inhibited GC cell proliferation, migration, and invasion, confirming its oncogenic role.
- AFAP1-AS1 directly interacts with miR-155-5p and regulates FGF7 expression, promoting GC cell proliferation and metastasis via the miR-155-5p/FGF7 axis.
Conclusions:
- AFAP1-AS1 functions as an oncogenic long non-coding RNA (lncRNA) in GC progression.
- AFAP1-AS1 acts as a competing endogenous RNA (ceRNA), sponging miR-155-5p to upregulate FGF7.
- AFAP1-AS1 represents a potential novel therapeutic target for gastric cancer.
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