Circ-0001313/miRNA-510-5p/AKT2 axis promotes the development and progression of colon cancer

Related Experiment Video

Updated: Dec 28, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.9K

Fang-Ling Tu1, Xi-Qing Guo2, Hai-Xia Wu3

  • 1Department of General Medicine, Chongqing Three Gorges Central Hospital Chongqing, China.

American Journal of Translational Research
|February 14, 2020
PubMed

Insights

Circular RNAs (circRNAs) are key in colon cancer. This study shows circ-0001313 promotes colon cancer by sponging miRNA-510-5p, upregulating AKT2, and impacting cell proliferation and apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, yet their specific roles in colon cancer progression are not fully understood.
  • Understanding circRNA expression and function is vital for developing novel therapeutic strategies against colon cancer.

Purpose of the Study:

  • To investigate the expression pattern and biological function of circ-0001313 in colon cancer.
  • To elucidate the molecular mechanism by which circ-0001313 influences colon cancer progression through the miRNA-510-5p/AKT2 axis.

Main Methods:

  • CircRNA microarray analysis to identify differentially expressed circRNAs in colon cancer.
  • Quantitative real-time PCR (qRT-PCR) to determine expression levels of circ-0001313 and miRNA-510-5p.
  • Bioinformatics prediction, dual-luciferase reporter gene assay, and western blot to confirm molecular interactions and pathway activation.
  • EdU assay and flow cytometry to evaluate cellular proliferation and apoptosis.

Main Results:

  • Circ-0001313 was significantly upregulated in colon cancer tissues and cell lines.
  • Knockdown of circ-0001313 inhibited colon cancer cell proliferation and induced apoptosis.
  • Circ-0001313 acts as a molecular sponge for miRNA-510-5p, leading to the upregulation of its target gene, AKT2.
  • Circ-0001313 influences the expression of Bcl-2 family proteins and activates the PI3K/Akt signaling pathway.

Conclusions:

  • Circ-0001313 plays a crucial role in the pathogenesis of colon cancer.
  • The circ-0001313/miRNA-510-5p/AKT2 axis represents a potential therapeutic target for colon cancer treatment.
Keywords:
AKT2Colon cancercirc-0001313miRNA-510-5p

More Related Videos

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.1K
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

5.9K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K