[Nuclear DNA pattern and various prognostic factors of the gallbladder cancer]

Nuclear DNA patterns which was obtained by the Feulgen staining and microspectrophotometric measurement in the gallbladder cancer were analyzed. DNA score was determined by the peak ploidy unit and decided numbers of frequency in each ploidy unit and compared with the various factors influencing prognosis of the patients. DNA score was significantly lower in cancer with papillary form and limited involvement to the mucosa compared with that with other forms and involvement beyond the proper muscle layer. DNA score was also significantly lower in cancer without hepatic infiltration, vascular invasion and lymph node metastasis than that with those characteristics. In experimental studies VX2 cancer cells which were implanted in the gallbladder subserosa in rabbits, DAN score did not show any changes with the growth of carcinoma implanted. Biologic nature of the tumor which was evaluated with nuclear DNA pattern of cancer cells was fundamental to define the spread of carcinoma and the prognosis of the patients.