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Related Experiment Video

Updated: Dec 28, 2025

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Published on: April 22, 2017

Maternal Larp6 controls oocyte development, chorion formation and elevation.

Hoi Ting A Hau¹, Oluwaseun Ogundele¹, Andrew H Hibbert¹

¹Randall Centre for Cell and Molecular Biophysics, New Hunt's House, Guy's Campus, King's College London, London SE1 1UL, UK.

Development (Cambridge, England)

|February 15, 2020

Summary

This summary is machine-generated.

La-related protein 6 (Larp6) proteins are crucial for zebrafish egg development. Zebrafish mutants lacking Larp6 show defective egg chorion formation, impacting egg quality and activation.

Keywords:

Chorion Knockout Larp6 Mass spectrometry Maternal effect Oocyte Zebrafish

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Area of Science:

Developmental Biology
Molecular Genetics
Reproductive Biology

Background:

La-related protein 6 (Larp6) is a conserved RNA-binding protein involved in various cellular processes.
Zebrafish possess two Larp6 family genes: larp6a and larp6b.
Previous studies suggested Larp6 roles in collagen biogenesis, muscle development, and cell proliferation.

Purpose of the Study:

To investigate the function of Larp6 proteins in zebrafish development.
To determine the specific roles of larp6a and larp6b in oogenesis and egg quality.
To elucidate the molecular mechanisms underlying Larp6-mediated chorion formation.

Main Methods:

Generation and analysis of single and double homozygous larp6a and larp6b zebrafish mutants.
Phenotypic assessment of muscle structure, fertility, and egg viability.
Detailed examination of egg chorion structure using electron microscopy.
Mass spectrometry to analyze chorion protein composition.

Main Results:

Zygotic larp6a and larp6b mutants showed no defects in muscle structure or overall viability and fertility.
larp6a mutant females produced eggs with fragile and incompletely elevated chorions.
Double larp6a;larp6b mutants exhibited more severe chorion defects than single mutants.
Electron microscopy revealed impaired chorionogenesis during oocyte development.
Mass spectrometry identified significant reductions in zona pellucida and lectin-type proteins in mutant chorions.

Conclusions:

Larp6 proteins are essential for normal zebrafish oocyte development and chorion formation.
Larp6 proteins play a critical role in regulating the composition of egg chorion proteins.
Defects in chorion formation mediated by Larp6 impact egg activation.