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Whole-Genome Sequence of Fish-Pathogenic Enterococcus faecalis Strain BFFF11
Tasmina Akter1, M Mahbubur Rahman1, Alfred Chin Yen Tay2
1Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh tasmina.fm@gmail.com mahbub-biotech@bsmrau.edu.bd.
A novel strain of the fish pathogen Enterococcus faecalis, BFFF11, was identified in tilapia with streptococcosis in Bangladesh. Its complete genome sequence provides insights into fish disease mechanisms.
Area of Science:
- Microbiology
- Aquatic Animal Health
- Genomics
Background:
- Streptococcosis is a significant disease affecting tilapia aquaculture.
- Enterococcus faecalis is an emerging fish pathogen.
- Disease outbreaks in aquaculture necessitate pathogen identification and characterization.
Purpose of the Study:
- To isolate and identify the causative agent of streptococcosis in tilapia.
- To perform whole-genome sequencing of the isolated Enterococcus faecalis strain.
- To provide genomic data for understanding fish-pathogenic bacteria.
Main Methods:
- Bacterial isolation and identification from diseased tilapia.
- Phenotypic and biochemical characterization of the isolate.
- Whole-genome sequencing and analysis of the bacterial strain.
Main Results:
- Enterococcus faecalis strain BFFF11 was isolated from diseased tilapia in Bangladesh.
- The whole genome of strain BFFF11 comprises 3,067,042 base pairs.
- The GC content of the BFFF11 genome was determined to be 37.4%.
Conclusions:
- Enterococcus faecalis strain BFFF11 is a fish-pathogenic bacterium.
- The genomic information of BFFF11 can aid in developing diagnostic and control strategies for streptococcosis.
- This study contributes valuable genomic data for aquatic veterinary microbiology.
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