Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

511
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
511

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tracking active heterotrophic microbial communities in the Guaymas Basin deep biosphere with BONCAT-FACS.

ISME communications·2026
Same author

Proteomic stress response by a novel methanogen enriched from the Great Salt Lake.

Microbiology spectrum·2026
Same author

Ecology of methyl-coenzyme M reductase encoding Thermoproteota.

Current opinion in microbiology·2025
Same author

Cultivation of Methanonezhaarchaeia, the third class of methanogens within the phylum Thermoproteota.

Science advances·2025
Same author

Seismic shifts in the geochemical and microbial composition of a Yellowstone aquifer.

PNAS nexus·2025
Same author

A collection of archaeal 16S rRNA Clone-FISH cultures for probe validation in fluorescence <i>in situ</i> hybridization experiments.

Microbiology resource announcements·2025

Related Experiment Video

Updated: Dec 28, 2025

Characterizing Microbiome Dynamics &#8211; Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

12.4K

Next-generation physiology approaches to study microbiome function at single cell level.

Roland Hatzenpichler1, Viola Krukenberg2, Rachel L Spietz2

  • 1Department of Chemistry and Biochemistry, Center for Biofilm Engineering, and Thermal Biology Institute, Montana State University, Bozeman, MT, USA. roland.hatzenpichler@montana.edu.

Nature Reviews. Microbiology
|February 15, 2020
PubMed
Summary
This summary is machine-generated.

Next-generation physiology approaches offer non-destructive ways to study cellular function in native environments. These methods enhance our understanding of microbiome interactions and their impact on ecosystems.

More Related Videos

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.5K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

1.3K

Related Experiment Videos

Last Updated: Dec 28, 2025

Characterizing Microbiome Dynamics &#8211; Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

12.4K
Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.5K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

1.3K

Area of Science:

  • Microbial Ecology
  • Cellular Physiology
  • Environmental Microbiology

Background:

  • Cellular functions in native environments are difficult to predict from genomic data or isolated studies.
  • Traditional microbiome research methods are often destructive, lack spatial resolution, or have low throughput.
  • Understanding microbial interactions and their environmental impact is crucial.

Purpose of the Study:

  • To review next-generation physiology approaches for studying microbiome function.
  • To highlight how these new technologies provide deeper insights into cellular behavior in situ.
  • To discuss the complementary nature of non-destructive analysis and cell separation techniques.

Main Methods:

  • Overview of recently developed next-generation physiology technologies.
  • Emphasis on non-destructive analysis of cellular phenotypes.
  • Integration of cell separation for downstream analyses.

Main Results:

  • Next-generation physiology enables non-destructive, high-resolution study of microbial communities.
  • Combined approaches of phenotype analysis and cell sorting offer powerful insights.
  • These methods overcome limitations of traditional experimental techniques.

Conclusions:

  • Next-generation physiology approaches are transforming microbial ecology.
  • Widespread application will significantly improve understanding of microorganism function in native habitats.
  • These advancements are key to unraveling complex microbial ecosystem dynamics.