Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA ploidy in thin melanoma.

I M Zardawi1, L Jarvis, P Hersey

  • 1Discipline of Pathology, Faculty of Medicine, University of Newcastle.

Pathology
|July 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crust Composition and the Shallow Heat Source in KS 1731-260.

Physical review letters·2025
Same author

Ergogenic effects of a 10-day L-citrulline supplementation on time to exhaustion and cardiorespiratory and metabolic responses in healthy individuals: a double-blind, randomised, placebo-controlled crossover trial.

Frontiers in sports and active living·2025
Same author

Correction: Repression of microRNA-768-3p by MEK/ERK signalling contributes to enhanced mRNA translation in human melanoma.

Oncogene·2025
Same author

Editorial Expression of Concern: Ets-1 mediates upregulation of Mcl-1 downstream of XBP-1 in human melanoma cells upon ER stress.

Oncogene·2025
Same author

Editorial Expression of Concern: Temozolomide induces senescence but not apoptosis in human melanoma cells.

British journal of cancer·2024
Same author

Mechanical circulatory support as a bridge to delivery in a pregnant patient with dilated cardiomyopathy and acute heart failure: a case report.

International journal of obstetric anesthesia·2024

DNA ploidy analysis using nuclear area and optical density showed differences between benign nevi and melanomas. Further research is needed to confirm if these DNA ploidy characteristics can reliably distinguish between non-metastasizing and metastasizing melanomas.

Area of Science:

  • Oncology
  • Dermatopathology
  • Molecular Pathology

Background:

  • Distinguishing between benign nevi, thin non-metastasizing melanomas (TNM), and thin metastasizing melanomas (TMM) is crucial for patient prognosis.
  • DNA ploidy analysis is a potential tool for characterizing cellular atypia and predicting melanoma behavior.

Purpose of the Study:

  • To investigate DNA ploidy characteristics, specifically nuclear area (NA) and nuclear integrated optical density (IOD), in benign nevi (BN), TNM, and TMM.
  • To determine if NA and IOD can differentiate between these three types of skin lesions.

Main Methods:

  • Utilized an image analyzer to measure nuclear area (NA) and nuclear integrated optical density (IOD) in DNA ploidy analysis.
  • Compared NA and IOD measurements across samples of BN, TNM, and TMM.

Related Experiment Videos

Main Results:

  • Significant differences in nuclear area (NA) were observed between nevus cells and melanoma cells.
  • Significant differences in nuclear integrated optical density (IOD) were found between TNM and TMM samples.
  • While differences in NA and IOD were detected across all three groups (BN, TNM, TMM), substantial intra-sample and intra-group variability was noted.

Conclusions:

  • Nuclear area and nuclear integrated optical density show potential in distinguishing between benign nevi and melanomas, and between different subtypes of melanoma.
  • The observed variability necessitates further studies to establish the diagnostic utility of these DNA ploidy parameters for accurate classification of individual skin lesions.